This study presents an integrated environmental assessment of the Danube River at three sites near Novi Sad, Serbia, combining physicochemical water quality analysis with acoustic soundscape monitoring. Water samples collected at Burma Beach, Estuary DTD, and Resort Ribarsko Ostrvo in September 2024 were analyzed against Serbian and WHO standards. The results revealed overall high ecological status, with most parameters-including dissolved oxygen, nitrates, nitrites, and phosphorus-falling within Class I limits. However, localized exceedances in total organic carbon (TOC), ammonium, arsenic, and copper placed some samples in Class II and III, indicating moderate pressures from urban and industrial activities. Acoustic monitoring enriched the evaluation by capturing distinctive environmental soundscapes, from bird colonies and recreational noise to industrial ship traffic and underwater disturbances. These findings highlight the Danube's resilience while emphasizing the need for continuous monitoring to manage localized stressors. By combining scientific and sensory dimensions, this study demonstrates a novel interdisciplinary approach to river assessment and public environmental engagement.