Water contamination represents a critical global challenge, demanding safe and efficient purification technologies. Conventional disinfection methods such as chlorination, ozonation, and UV irradiation are limited by high energy demand and toxic by-products. In this study, a novel nanocomposite material, three-dimensional copper foam modified with Ag nanoparticles and CuO nanowires, was investigated for antibacterial activity. The material demonstrated strong efficiency in removing Escherichia coli and Staphylococcus aureus, achieving up to 99.9% removal under optimized conditions. Results indicate that bacterial elimination improves with voltage intensity and decreases with flow rate, confirming the nanocomposite's potential for practical water purification applications.