Background: Whooping cough is an acute infectious disease caused by the Bordetella pertussis bacteria. Increase in the number of infections caused by this bacterium has been noticed in many countries, even in those with high vaccination rates. Proper diagnostics are crucial for adequate treatment and prevention of further spreading of infection. Methods andObjectives: 2285 nasopharyngeal swabs have been analyzed. All samples were collected in COPAN eSwab media. Sampling was carried out at the Institute of Public Health of Serbia "Dr Milan Jovanović Batut, health centers and hospitals. VIASURE Bordetella Real Time PCR Detection Kit (CerTest Biotec), B.pertussis/parapertussis Genotyping Kit (Anatolia geneworks) (detecting the IS481 insertion sequence region), as well as RealBest DNA Bordetella species/Bordetella pertussis/ Bordetella bronchiseptica Kit (Vector Best) (detecting 16S rRNA and FIM3 genes) have been used. The aim of this study is an overview of the results obtained using molecular tests of three different manufacturers during the epidemic in the period June 2023-June 2024. Results: By analyzing 2285 samples, using the mentioned tests, it was observed that 25.4% of the samples were positive. The largest number of patients was tested in December 2023, with 29% of positive cases detected. Conclusions: Real time PCR tests for Bordetella pertussis enable time-efficient and reliable diagnosis, which is of great importance in conditions of increased number of samples. Increased sensitivity and speed of analysis are crucial for patients with borderline positive values, but also for unvaccinated and newborns, for whom infection can have a lethal effect.