Diflubenzuron is an insecticide from the group of insect growth regulators, widely used in agriculture and forestry for pest control. Its application can have negative effects on non-target organisms and ecosystems. In the environment, diflubenzuron degrades through hydrolysis, photodegradation, and microbial activity, producing 4-chloroaniline (4-CA), a toxic and persistent byproduct. In aquatic ecosystems, diflubenzuron disrupts chitin synthesis, endangering crustaceans, mollusks, and planktonic organisms, whose growth and development depends on this polysaccharide. While it does not directly affect fish, 4-CA can have hematotoxic and genotoxic effects. Diflubenzuron can adversely impact bees, particularly larvae, thereby threatening plant pollination. Additionally, it can harm beneficial predators including ladybugs, lacewings, and parasitic wasps, which naturally regulate pest populations. In soil, diflubenzuron reduces the activity of earthworms and microorganisms, potentially leading to soil fertility degradation. There is also a risk of groundwater contamination by its metabolites, which can persist in the ecosystem for extended periods. Although diflubenzuron is effective in pest control, its application requires careful management to minimize negative effects on non-target organisms and preserve ecological balance. Further research is needed to assess the long-term consequences of its use.